Evaluation of Xylella fastidiosa genetic diversity by fAFPL markers
نویسندگان
چکیده
منابع مشابه
Evaluation of the Genetic Diversity of Xylella fastidiosa Strains from Citrus and Coffee Hosts by Single-Nucleotide Polymorphism Markers.
ABSTRACT The aim of this study was to obtain information about genetic diversity and make some inferences about the relationship of 27 strains of Xylella fastidiosa from different hosts and distinct geographical areas. Single-nucleotide polymorphism (SNP) molecular markers were identified in DNA sequences from 16 distinct regions of the genome of 24 strains of X. fastidiosa from coffee and citr...
متن کاملMultilocus simple sequence repeat markers for differentiating strains and evaluating genetic diversity of Xylella fastidiosa.
A genome-wide search was performed to identify simple sequence repeat (SSR) loci among the available sequence databases from four strains of Xylella fastidiosa (strains causing Pierce's disease, citrus variegated chlorosis, almond leaf scorch, and oleander leaf scorch). Thirty-four SSR loci were selected for SSR primer design and were validated in PCR experiments. These multilocus SSR primers, ...
متن کاملMULTI-LOCUS SIMPLE SEQUENCE REPEAT (SSR) MARKERS FOR GENOTYPING AND ASSESSING GENETIC DIVERSITY OF XYLELLA FASTIDIOSA IN CALIFORNIA Project Leader:
We have designed and developed Xylella fastidiosa (Xf) Simple Sequence Repeat (SSR) primers. Thirty-four of them have been validated and are available to public (Lin et al. 2005). These primers are Xf-specific and powerful for detecting polymorphism among and within crop-associated Xf strains and can be used for Xf genotyping, population structure and genetic diversity studies. Recently, we use...
متن کاملEvaluation of genetic diversity of Avicenniaceae family in Indian sundarban by using RAPD and ISSR markers
The present investigation was undertaken to describe the relationships among species of Avicenniaceae family which have both economic and medicinal activities, collected in Indian Sundarban by the help of RAPD and ISSR markers. In this study, three different species of Avicennia genus were collected. Frozen young leaves (-200C) were taken to isolate the genomic DNA using a slightly modified CTA...
متن کاملGenetic Diversity of Xylella fastidiosa Strains from Costa Rica, São Paulo, Brazil, and United States.
ABSTRACT The diversity of 42 Xylella fastidiosa strains from Costa Rica, São Paulo, Brazil, and the United States were analyzed using the sequence of the 16S rRNA gene by variable number of tandem repeat (VNTR) fragment analysis and by restriction fragment length polymorphisms (RFLP) of a specific polymerase chain reaction (PCR)-amplification product using enzyme CfoI. Limited variability in th...
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ژورنال
عنوان ژورنال: Revista Brasileira de Fruticultura
سال: 2008
ISSN: 0100-2945
DOI: 10.1590/s0100-29452008000100037